We are very grateful and happy to have a PhD student Maria Escrivá Conde from SU Stockholm on hands-on training at NIB. Maria came to train NIB researchers to set up the microscopy settings for In Situ Sequencing (ISS) methodology. Planning joint activities further plans for collaborative experiments focused around e.g. ISS methodology and spatial analysis of the generated glioblastoma organoids were also discussed.

Maria is exploring the methodology and significance of analysing In Situ Sequencing (ISS) data in the context of colorectal cancer liver metastasis (CRCLM) and glioblastoma (GBM) samples. ISS offers a powerful approach to investigate spatially resolved gene expression patterns within tissues, providing crucial insights into tumour microenvironments and cellular interactions. She discussed the principles of ISS data analysis. Additionally, she delved into the specific applications of ISS in understanding the molecular mechanisms underlying colorectal cancer liver metastasis and glioblastoma progression. By harnessing ISS technology, researchers can uncover novel biomarkers, therapeutic targets, and spatially defined gene expression signatures, ultimately advancing our understanding and treatment strategies for these aggressive malignancies.

Thank you Maria to share all your knowledge with us and to boost us with ISS energy😊.

We will continue to do our best together to uncover more about the biology of this highly malignant tumours.