In October, our PhD student Simona Katrin Galun from the Department of genetic toxicology and cancer biology at the National institute of biology (NIB) visited Science for life laboratory in Sweden. As part of her one-month hands-on training, she had the opportunity to collaborate with Mats Nilsson’s group to learn the in situ sequencing technique developed in their lab. In situ sequencing is a powerful spatial transcriptomics method that allows for the visualization and analysis of gene expression patterns directly within intact tissues. This technique has great potential for advancing cancer research, particularly in understanding tumors and their microenvironment. Over the past month, Katrin has gained both theoretical knowledge and hands-on experience with the in situ sequencing protocol, enabling her to independently analyze human glioblastoma tissue samples and explore the immune microenvironment of tumors. She looks forward to applying this method in our own lab to further advance our research.A special thanks to Chika Yokota, head of the ISS department, for her guidance and sharing invaluable expertise. We would also like to express our gratitude to Maria Escriva Conde for mentorship during data analysis, and to all the members of Nilsson’s lab for fostering such a welcoming and collaborative environment.