The National Institute of Biology hosted a Three-Day Confocal Microscopy Workshop: Theory Meets Hands-On Learning with Assist. Prof. Miloš Vittori. Participants from the Department of Genetic Toxicology and Cancer Biology, involved in the CutCancer project, delved into practical sessions, operating the cutting-edge Lyca Stellaris 8 microscope with the Light sheet technology. The workshop showcased live and fixed spheroid staining and imaging, immunocytochemically labelled spheroid section imaging and emphasized the significance of image acquisition and analysis techniques. A dynamic Q&A session and shared experiences further enriched the collaborative learning environment. The workshop was fruitful and successful, leaving participants equipped with enhanced skills and practical insights for their research endeavours.